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hcc cell lines (catalogue number: crl-8024tm)  (BioResource International Inc)

 
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    BioResource International Inc hcc cell lines (catalogue number: crl-8024tm)
    a The diagram illustrates how miR424 was identified from the miRNA sequencing data and predicted by Venn screening from miRNA database websites. b Sequence alignment of the human miR424 seed sequence with the 3’-UTR of CBX4. The mutated sequence in the matched binding sites for the gene that was used to create the firefly luciferase reporter constructs is shown at the bottom of the gene set. A luciferase reporter assay demonstrated that miR424 inhibited the transcription of the wild-type but not the mutant 3’-UTRs of CBX4. c The expression of endogenous CBX4 was inhibited in miR424-overexpressed Huh7-SR and <t>PLC-SR</t> cells. d In contrast, CBX4 levels were increased in Huh7 and PLC cells with miR424-TUD. All data were compared with the respective controls, and the mRNA level was detected by qRT-PCR. CBX4 mRNA expression was normalised to that of GAPDH mRNA; and three independent experiments were conducted. e Relative miR424 expression in <t>HCC</t> tissues and matched adjacent normal tissues as assessed by qRT-PCR. f , g Relative expression data of miR424 in HCC cases were further analysed. The negative relationship between miR424 expression and liver cirrhosis ( f ) and size ( g ). h , i Kaplan–Meier curves of disease-free survival (DFS) ( h ) and overall survival (OS) ( i ). Survival of the high and low miR424 expression groups assessed using log-rank (Mantel–Cox) test in HCC, which were divided according to a cut-off of 2.5, the median value of CBX4 mRNA expression relative to GAPDH mRNA. j From the TCGA database, tumour purity was highly negatively correlated with miR424 expression. k Linear regression and correlation between the miR424 and CBX4 mRNA levels in 341 HCC tissues from the TCGA database. l The negative linear regression and correlation analysis for the relation of the mRNA levels of CBX4 and those of miR424 in 106 HCC patients by qRT-PCR.
    Hcc Cell Lines (Catalogue Number: Crl 8024tm), supplied by BioResource International Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hcc cell lines (catalogue number: crl-8024tm)/product/BioResource International Inc
    Average 90 stars, based on 1 article reviews
    hcc cell lines (catalogue number: crl-8024tm) - by Bioz Stars, 2026-03
    90/100 stars

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    1) Product Images from "Inhibiting CBX4 efficiently protects hepatocellular carcinoma cells against sorafenib resistance"

    Article Title: Inhibiting CBX4 efficiently protects hepatocellular carcinoma cells against sorafenib resistance

    Journal: British Journal of Cancer

    doi: 10.1038/s41416-020-01240-6

    a The diagram illustrates how miR424 was identified from the miRNA sequencing data and predicted by Venn screening from miRNA database websites. b Sequence alignment of the human miR424 seed sequence with the 3’-UTR of CBX4. The mutated sequence in the matched binding sites for the gene that was used to create the firefly luciferase reporter constructs is shown at the bottom of the gene set. A luciferase reporter assay demonstrated that miR424 inhibited the transcription of the wild-type but not the mutant 3’-UTRs of CBX4. c The expression of endogenous CBX4 was inhibited in miR424-overexpressed Huh7-SR and PLC-SR cells. d In contrast, CBX4 levels were increased in Huh7 and PLC cells with miR424-TUD. All data were compared with the respective controls, and the mRNA level was detected by qRT-PCR. CBX4 mRNA expression was normalised to that of GAPDH mRNA; and three independent experiments were conducted. e Relative miR424 expression in HCC tissues and matched adjacent normal tissues as assessed by qRT-PCR. f , g Relative expression data of miR424 in HCC cases were further analysed. The negative relationship between miR424 expression and liver cirrhosis ( f ) and size ( g ). h , i Kaplan–Meier curves of disease-free survival (DFS) ( h ) and overall survival (OS) ( i ). Survival of the high and low miR424 expression groups assessed using log-rank (Mantel–Cox) test in HCC, which were divided according to a cut-off of 2.5, the median value of CBX4 mRNA expression relative to GAPDH mRNA. j From the TCGA database, tumour purity was highly negatively correlated with miR424 expression. k Linear regression and correlation between the miR424 and CBX4 mRNA levels in 341 HCC tissues from the TCGA database. l The negative linear regression and correlation analysis for the relation of the mRNA levels of CBX4 and those of miR424 in 106 HCC patients by qRT-PCR.
    Figure Legend Snippet: a The diagram illustrates how miR424 was identified from the miRNA sequencing data and predicted by Venn screening from miRNA database websites. b Sequence alignment of the human miR424 seed sequence with the 3’-UTR of CBX4. The mutated sequence in the matched binding sites for the gene that was used to create the firefly luciferase reporter constructs is shown at the bottom of the gene set. A luciferase reporter assay demonstrated that miR424 inhibited the transcription of the wild-type but not the mutant 3’-UTRs of CBX4. c The expression of endogenous CBX4 was inhibited in miR424-overexpressed Huh7-SR and PLC-SR cells. d In contrast, CBX4 levels were increased in Huh7 and PLC cells with miR424-TUD. All data were compared with the respective controls, and the mRNA level was detected by qRT-PCR. CBX4 mRNA expression was normalised to that of GAPDH mRNA; and three independent experiments were conducted. e Relative miR424 expression in HCC tissues and matched adjacent normal tissues as assessed by qRT-PCR. f , g Relative expression data of miR424 in HCC cases were further analysed. The negative relationship between miR424 expression and liver cirrhosis ( f ) and size ( g ). h , i Kaplan–Meier curves of disease-free survival (DFS) ( h ) and overall survival (OS) ( i ). Survival of the high and low miR424 expression groups assessed using log-rank (Mantel–Cox) test in HCC, which were divided according to a cut-off of 2.5, the median value of CBX4 mRNA expression relative to GAPDH mRNA. j From the TCGA database, tumour purity was highly negatively correlated with miR424 expression. k Linear regression and correlation between the miR424 and CBX4 mRNA levels in 341 HCC tissues from the TCGA database. l The negative linear regression and correlation analysis for the relation of the mRNA levels of CBX4 and those of miR424 in 106 HCC patients by qRT-PCR.

    Techniques Used: Sequencing, Binding Assay, Luciferase, Construct, Reporter Assay, Mutagenesis, Expressing, Quantitative RT-PCR



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    hcc cell lines (catalogue number: crl-8024tm)  (BioResource International Inc)
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    BioResource International Inc hcc cell lines (catalogue number: crl-8024tm)
    a The diagram illustrates how miR424 was identified from the miRNA sequencing data and predicted by Venn screening from miRNA database websites. b Sequence alignment of the human miR424 seed sequence with the 3’-UTR of CBX4. The mutated sequence in the matched binding sites for the gene that was used to create the firefly luciferase reporter constructs is shown at the bottom of the gene set. A luciferase reporter assay demonstrated that miR424 inhibited the transcription of the wild-type but not the mutant 3’-UTRs of CBX4. c The expression of endogenous CBX4 was inhibited in miR424-overexpressed Huh7-SR and <t>PLC-SR</t> cells. d In contrast, CBX4 levels were increased in Huh7 and PLC cells with miR424-TUD. All data were compared with the respective controls, and the mRNA level was detected by qRT-PCR. CBX4 mRNA expression was normalised to that of GAPDH mRNA; and three independent experiments were conducted. e Relative miR424 expression in <t>HCC</t> tissues and matched adjacent normal tissues as assessed by qRT-PCR. f , g Relative expression data of miR424 in HCC cases were further analysed. The negative relationship between miR424 expression and liver cirrhosis ( f ) and size ( g ). h , i Kaplan–Meier curves of disease-free survival (DFS) ( h ) and overall survival (OS) ( i ). Survival of the high and low miR424 expression groups assessed using log-rank (Mantel–Cox) test in HCC, which were divided according to a cut-off of 2.5, the median value of CBX4 mRNA expression relative to GAPDH mRNA. j From the TCGA database, tumour purity was highly negatively correlated with miR424 expression. k Linear regression and correlation between the miR424 and CBX4 mRNA levels in 341 HCC tissues from the TCGA database. l The negative linear regression and correlation analysis for the relation of the mRNA levels of CBX4 and those of miR424 in 106 HCC patients by qRT-PCR.
    Hcc Cell Lines (Catalogue Number: Crl 8024tm), supplied by BioResource International Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hcc cell lines (catalogue number: crl-8024tm)/product/BioResource International Inc
    Average 90 stars, based on 1 article reviews
    hcc cell lines (catalogue number: crl-8024tm) - by Bioz Stars, 2026-03
    90/100 stars
    		  Buy from Supplier

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    a The diagram illustrates how miR424 was identified from the miRNA sequencing data and predicted by Venn screening from miRNA database websites. b Sequence alignment of the human miR424 seed sequence with the 3’-UTR of CBX4. The mutated sequence in the matched binding sites for the gene that was used to create the firefly luciferase reporter constructs is shown at the bottom of the gene set. A luciferase reporter assay demonstrated that miR424 inhibited the transcription of the wild-type but not the mutant 3’-UTRs of CBX4. c The expression of endogenous CBX4 was inhibited in miR424-overexpressed Huh7-SR and PLC-SR cells. d In contrast, CBX4 levels were increased in Huh7 and PLC cells with miR424-TUD. All data were compared with the respective controls, and the mRNA level was detected by qRT-PCR. CBX4 mRNA expression was normalised to that of GAPDH mRNA; and three independent experiments were conducted. e Relative miR424 expression in HCC tissues and matched adjacent normal tissues as assessed by qRT-PCR. f , g Relative expression data of miR424 in HCC cases were further analysed. The negative relationship between miR424 expression and liver cirrhosis ( f ) and size ( g ). h , i Kaplan–Meier curves of disease-free survival (DFS) ( h ) and overall survival (OS) ( i ). Survival of the high and low miR424 expression groups assessed using log-rank (Mantel–Cox) test in HCC, which were divided according to a cut-off of 2.5, the median value of CBX4 mRNA expression relative to GAPDH mRNA. j From the TCGA database, tumour purity was highly negatively correlated with miR424 expression. k Linear regression and correlation between the miR424 and CBX4 mRNA levels in 341 HCC tissues from the TCGA database. l The negative linear regression and correlation analysis for the relation of the mRNA levels of CBX4 and those of miR424 in 106 HCC patients by qRT-PCR.

    Journal: British Journal of Cancer

    Article Title: Inhibiting CBX4 efficiently protects hepatocellular carcinoma cells against sorafenib resistance

    doi: 10.1038/s41416-020-01240-6

    Figure Lengend Snippet: a The diagram illustrates how miR424 was identified from the miRNA sequencing data and predicted by Venn screening from miRNA database websites. b Sequence alignment of the human miR424 seed sequence with the 3’-UTR of CBX4. The mutated sequence in the matched binding sites for the gene that was used to create the firefly luciferase reporter constructs is shown at the bottom of the gene set. A luciferase reporter assay demonstrated that miR424 inhibited the transcription of the wild-type but not the mutant 3’-UTRs of CBX4. c The expression of endogenous CBX4 was inhibited in miR424-overexpressed Huh7-SR and PLC-SR cells. d In contrast, CBX4 levels were increased in Huh7 and PLC cells with miR424-TUD. All data were compared with the respective controls, and the mRNA level was detected by qRT-PCR. CBX4 mRNA expression was normalised to that of GAPDH mRNA; and three independent experiments were conducted. e Relative miR424 expression in HCC tissues and matched adjacent normal tissues as assessed by qRT-PCR. f , g Relative expression data of miR424 in HCC cases were further analysed. The negative relationship between miR424 expression and liver cirrhosis ( f ) and size ( g ). h , i Kaplan–Meier curves of disease-free survival (DFS) ( h ) and overall survival (OS) ( i ). Survival of the high and low miR424 expression groups assessed using log-rank (Mantel–Cox) test in HCC, which were divided according to a cut-off of 2.5, the median value of CBX4 mRNA expression relative to GAPDH mRNA. j From the TCGA database, tumour purity was highly negatively correlated with miR424 expression. k Linear regression and correlation between the miR424 and CBX4 mRNA levels in 341 HCC tissues from the TCGA database. l The negative linear regression and correlation analysis for the relation of the mRNA levels of CBX4 and those of miR424 in 106 HCC patients by qRT-PCR.

    Article Snippet: The human HCC cell lines PLC (catalogue number: CRL-8024TM) was obtained from the Global Bioresource Center of American Type Culture Collection (ATCC) and Huh7 (catalogue number: JCRB0403) was purchased from Health Science Research Resources Bank (Osaka, Japan).

    Techniques: Sequencing, Binding Assay, Luciferase, Construct, Reporter Assay, Mutagenesis, Expressing, Quantitative RT-PCR